منابع مشابه
Against a whole-genome shotgun.
The human genome project is entering its decisive final phase, in which the genome sequence will be determined in large-scale efforts in multiple laboratories worldwide. A number of sequencing groups are in the process of scaling up their throughput; over the next few years they will need to attain a collective capacity approaching half a gigabase per year to complete the 3-Gb genome sequence b...
متن کاملWhole Genome Shotgun
The motivation for sequencing genomes is rather direct. According to the central dogma of biology, elements of the genome are translated via several mechanisms into the structure and function of organisms. But in order to study genomes, we must first sequence them. Whole Genome Shotgun (WGS) is a successful method for sequencing genomes. This lecture we will discuss how WGS works, some of the c...
متن کاملHuman whole-genome shotgun sequencing.
Large-scale sequencing of the human genome is now under way (Boguski et al. 1996; Marshall and Pennisi 1996). Although at the beginning of the Genome Project, many doubted the scientific value of sequencing the entire human genome, these doubts have evaporated almost entirely (Gibbs 1995; Olson 1995). Primary reasons for generating the human genomic sequence are listed in Table 1. The approach ...
متن کاملARACHNE: a whole-genome shotgun assembler.
We describe a new computer system, called ARACHNE, for assembling genome sequence using paired-end whole-genome shotgun reads. ARACHNE has several key features, including an efficient and sensitive procedure for finding read overlaps, a procedure for scoring overlaps that achieves high accuracy by correcting errors before assembly, read merger based on forward-reverse links, and detection of re...
متن کاملMapping whole genome shotgun sequence and variant
Genomics research in mammals has produced reference genome sequences that are essential for identifying variation associated with disease. High quality reference genome sequences are now available for humans, model species, and economically important agricultural animals. Comparisons between these species have provided unique insights into mammalian gene function. However, the number of speci...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1997
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.7.5.410